This controlled clinical trial was carried out in 2 phases in the Investigational Medicine Unit of National University Hospital (NUH), Singapore. Study procedures were accepted by the National Healthcare Group Domain Specific Review Board in July 2010 (Reference Number: 2010/00226), and written knowledgeable consent was obtained from all participants. Healthy girls with singleton pregnancies between 27 to 32 weeks gestation had been recruited on a voluntary foundation from amongst hospital workers and taobao usa clinic patients.

Eight pregnant health care staff were recruited in phases I and 20 pregnant ladies have been recruited in section II to participate within the examine. Subjects have been instructed to have sufficient rest and lolita dress to avoid strenuous activity previous to the study to make sure that the assessments have been conducted under regular way of life conditions. All topics were advised to have their meals not less than 2 h before begin of examine. A screening questionnaire was administered to every subject, who then had baseline medical and obstetric examinations previous to collaborating within the research.

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